Role Of Eea1 In Recycling Endosome Biogenesis And Cargo Sorting
The Endosomal-network begins at the plasma membrane following endocytosis by undergoing successive fusion resulting in maturation. The Rab effector proteins APPL1 and EEA1 drive endosomes towards the peri-nuclear cloud. The first part of the Ph.D. thesis establishes the role of APPL1 and dynein specific to endosome maturation. The second part addresses how multivalent EEA1 provides a physical basis for endosomal fusion and identifies its role in colocalization with Rab22a, which mediates Recycling-endosome biogenesis. A combination of live-cell imaging and super-resolution microscopy will be utilized to tease-apart dynamic details and molecular distributions of tightly regulated vectoral endosomal maturation.